This painting is an interpretation of the Golden Gate assembly technique that is used in molecular biology to assemble specific segments of DNA, in vitro, into a plasmid. This technique uses a Type IIS restriction enzyme, which cleaves the fragment of interest (dark green segment of the bridge) a few base pairs away from the recognition site (yellow areas on the bridge), resulting in sticky ends and allowing the segment to be inserted into the plasmid (circles in water) which is also digested with the same restriction enzyme. The plasmid can then be inserted into bacteria and this technique is used to clone a genomic segment.